The technique has an important role in mass production of planting material. The objective of this study was to obtain suitable in vitro propation of D.caryophillus L.cv. Maldives through induktion of adventitious shoot formulation, followed by multiplication of shoots, rooting and acclimatization. The study was conducted at tissue culture laboratory and glasshouseof IPPT, UPM, Malaysia from mei 2000 to November 2001.Three different types of explant (first and second young fully developed leaves and young internodus) and MS medium at five different media. Factorial experiment was arranged in randomized complete block design with four replications. Result of the experiment exhibited that the first young fully developed leaves and MS medium containing 0,1 mg/l BA and 0,01 mg/lNAA was the appropriate explant and concentration of BA and NAA to produce highest numbers of adventitious shoot.The concentration of BA and NAA was also suitable for preparing rooted shoots. Shoots were easily rooted on half-strength of MS media. Plantlets were simply acclimatized in a mixture medium of kossas peat and paddy charcoal (1:1 v/v) with 100% of survivability. Vigorous and healthy plantlets were also resulted in the medium. Abnormality of plants was 6% from total acclimatized-plantlets. The plantlets flowered at 4.5 to 5 months after acclimatization. The results expected can be used as a standard protocol in mass-production of carnation through adventitious shoot formation. Keyword; Dianthus caryophyllus; In vitro propagation; Adventitious shoot induction. Winarto,B.,M.A.Aziz A.A.Rashid, dan M.R. Ismail; Balai Penelitian Tanaman Hias Segunung
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