Micropropagation of temoe lawak in vitro has been conducted in Tissue Culture Laboratory Germplasm and Breeding Division at Research Institute for Spice and Medicinal Crops,Bogor from October 1999 to July 2000. Shoots of temoe lawak from Sukamulya Research Instalation were used as explants. Shoots were sterilized with sterilant such as 70 % alcohol , 0.2 % mercuri clorite , 20 % Clorox and then cleaned with aqudes before they are cultured. Murashige and Skoog (MS) which was added with vitamine from B group were used as basic medium. Several concentration of benzyl adenin i.e : 0; 0.5; 1.0; 1.5 and 2.0 mg/l singly and combination with NAA 0.5 mg/l were used as treatments. The treatment were arranged in Randomized Completely Design with three replications. Each replication consited of five bottles. The parameters observed were average the number and lengths of shoot , number of leaves and roots and the culture performance visually. The result showed that the greatest number of shoot and leaves were found by using 1.5 mg/ l BA during 8 weeks. The greatest roots were found on 1.0 mg/l BA + 0.5 mg/l NAA . BA used singly or combined with NAA could be used on micropropagation of temoe lawak in vitro. Key words : Curcuma xanthorhiza, BA, NAA, in vitro. Sitti Fatimah Syahid dan Endang Hadipoentyanti; Balai Penelitian Tanaman Rempah dan Obat.