Tuberose is normally propagated by the tuber. The smaller size of tuber the longer time plant to flower. The application of in vitro culture technique might be used for mass propagation. Up to know, the reasearch of in vitro culture of tuberose in Indonesia has not been reported. The objective of the study was to find out media formulation for organogenesis and embryogenesis. The experiments consisted of 4 steps of (1) shoot induction, (2) shoot multiplication, (3) induction of embryogenic callus, and (4) regeneration of embryogenic callus. The treatments for shoot induction were MS+BA 0 ppm, MS+BA 3 ppm, MS+BA 5 ppm and MS+BA 7 ppm. The shoots were multiplied on media MS+BA 7 ppm+glutamine 100 ppm, MS+BA 7 ppm, DKW+TDZ 7 ppm, and DKW+TDZ 7 ppm+glutamin 100 ppm, For induction of embryogenic callus, The treatments were MS+BA 2 ppm+TDZ 0,2 ppm, MS+BA 3 ppm+TDZ 0,4 ppm, MS+zeatin 1 ppm+ kinetiin 1ppm and MS+zeatin 0,5 ppm+kinetin 2 ppm. The results showed that the highest shoot formation was obtained from media MS+BA 3ppm but the earliest shoot initiation was obtained from media MS+BA0 ppm. The media formulation of MS+BA 7 ppm+glutamine 100 ppm gave the highest number of shoot and root. The application (18.75%) and high number of nodul (3.6) with the best visual calli. After subculturing, the highest number of nodul (17) was obtained from media MS+BA 2 ppm+TDZ 0.4 ppm. The embriogenic callus from media MS+zeatin 0.5 ppm+kinetin 2 ppm could develop to form somatic seed. Keywords: Polianthes tuberosa;Organogenesis: somatic embriogenesis;Media. Roostika,I. ,I.Mariska, dan R.Purnamaningsih; Balai besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
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